Nohut yanıklık etmeni Ascochyta rabiei (Pass) Labr.’nin Solanapyrone üretiminin saptanmasında spektrofotometrik yöntemin kullanılması

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Accumulation and Biosynthesis of Solanapyrone Phytotoxins by Ascochyta rabiei

The biosynthesis of the phytotoxins solanapyrone A , B and C produced by the phytopathogenic fungus Ascochyta rabiei has been investigated. To optimize feeding conditons for the tracer experiments the growth of the fungus and the accumulation of the toxins in submers culture were determined. The accumulation kinetics revealed that formation of the toxins occurs in the stationary phase of the gr...

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تعیین تنوع ژنتیکی قارچ عامل بیماری برق‌زدگی نخود [Ascochyta rabiei (Pass.) Lab.] ایران با استفاده از مارکرهای مولکولی RAPD

The poor information available on variation of Ascochyta blight fungus is the most important factor limiting chickpea breeding programs for resistance to blight disease. In this study, efforts were made to detect genetic variation of the pathogen in Iran. The RAPD marker was employed to evaluate 26 isolates collected from 16 provinces. Twelve random primers were used to analyze genomic DNA of t...

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Characterization and cloning of cutinase from Ascochyta rabiei.

Ascochyta rabiei, the causal agent of Ascochyta blight on chickpea plants, secretes a cutinase in the culture filtrate when it is induced by cutin or hydroxylated fatty acids. This cutinase is the main esterase in the culture fluids. The enzyme was purified to homogeneity by three successive chromatographic steps. It showed an apparent molecular weight of 22 kD in SDS-PAGE and cleaved ester bon...

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Mating Type Groups of Ascochyta rabiei (Teleomorph: Didymella rabiei), the Causal Agent of Chickpea Blight in Central Anatolia

The distribution of mating types of Ascochyta rabiei (teleomorph: Didymella rabiei) was studied on isolates collected from chickpea growing areas of Central Anatolia. Mating type assessments of 45 isolates from 6 different provinces were conducted under laboratory conditions. After keeping for 5-6 weeks, the pseudothecial development and ascospore production were observed on chickpea stem piece...

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Historical and contemporary multilocus population structure of Ascochyta rabiei (teleomorph: Didymella rabiei) in the Pacific Northwest of the United States.

The historical and contemporary population genetic structure of the chickpea Ascochyta blight pathogen, Ascochyta rabiei (teleomorph: Didymella rabiei), was determined in the US Pacific Northwest (PNW) using 17 putative AFLP loci, four genetically characterized, sequence-tagged microsatellite loci (STMS) and the mating type locus (MAT). A single multilocus genotype of A. rabiei (MAT1-1) was det...

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ژورنال

عنوان ژورنال: Tarım Bilimleri Dergisi

سال: 2007

ISSN: 1300-7580

DOI: 10.1501/tarimbil_0000000384